Simultaneous determination of retinol and alpha-tocopherol in serum or plasma by liquid chromatography.

نویسندگان

  • G L Catignani
  • J G Bieri
چکیده

Submitters: George L. Catignani, Department of Food Science, North Carolina State University, Raleigh, NC 27650 John G. Bieri, Nutritional Biochemistry Section, Laboratory of Nutrition and Endocrinology, National Institute of Arthritis, Metabolism, and Digestive Diseases, Bethesda, MD 20014 Evaluators: William J. Driskell and Mark M. Bashor, NutriLional Biochemistry Branch, Clinical Chemistry Division, Center for Environmental Health, Centers for Disease Control, Atlanta, GA 30333 Charles P. Turley and Marge A. Brewster, Metabolic Lab., Arkansas Childrens Hospital, Little Rock, AR 72201

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Simultaneous measurement of serum probucol and lipid-soluble antioxidants.

A method is described for the simultaneous measurement of probucol, retinol, tocopherols, lycopene, and carotenes by reverse phase high performance liquid chromatography. A high sensitivity was achieved by use of a microbore column and by monitoring the effluent at the optimum wavelengths of each substance with a diode array detector. The detection limits were lycopene 0.5 ng; alpha-carotene, b...

متن کامل

Simultaneous measurement of retinol, alpha-tocopherol and six carotenoids in human plasma by using an isocratic reversed-phase HPLC method.

A simple and sensitive isocratic reversed-phase high-performance liquid chromatography (HPLC) method for simultaneous determination of retinol, alpha-tocopherol and six carotenoids in human plasma was described. Sample preparation of the earlier published method was further developed by addition of ultrapure water, which enabled aqueous layer to freeze facilitating phase separation without pipe...

متن کامل

Ultra-performance liquid chromatographic determination of tocopherols and retinol in human plasma.

A rapid, selective and sensitive ultra-performance liquid chromatography method has been developed for the detection and quantification of tocopherols and retinol in human plasma. Alpha-tocopherol, gamma-tocopherol and retinol are assayed using fluorescence detection. Excitation/emission wavelengths are 295/330 nm and 325/470 nm for the analysis of both tocopherols and retinol, respectively. Re...

متن کامل

Simultaneous determination of retinol and α-tocopherol by high pressure liquid chromatography in micro-volumes of serum.

The lack of biochemical data to indicate the prevalence of marginal vitamin A deficiency (VAD) in the country is largely because of the difficulty of obtaining enough serum for analysis, especially from malnourished children. Efforts were therefore made to establish a high-pressure liquid chromatography system (HPLC) for the determination of retinol in micro-volumes of serum. Since our previous...

متن کامل

Rapid determination by reversed-phase high-performance liquid chromatography of Vitamins A and E in infant formulas.

A rapid, sensitive method has been developed for the simultaneous determination of retinol acetate, delta-, gamma-, alpha-tocopherol and alpha-tocopherol acetate. We compare two experimental procedures for simultaneous direct solvent extraction of these vitamins without previous saponification. Method I: the fat milk sample was extracted with ethanol-hexane and injected directly into the chroma...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Clinical chemistry

دوره 29 4  شماره 

صفحات  -

تاریخ انتشار 1983